Association of MTHFD1 G1958A, MTHFD1 T401C and CBS 844ins68bp with Breast Cancer in Jordan

Samira Daw Ameigaal¹, Almuthanna K. Alkaraki^2* and May Fouad Sadiq²

¹Department of Medical Laboratories, Higher Institute of Medical Sciences and Technology, Bani Waleed, Libya.

²Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid 21163, Jordan.

Corresponding Author E-mail: Alkaraki@yu.edu.jo

Abstract: MTHFD1 and CBS genes have key roles in folate and homocysteine metabolism. Many studies reported an association between cancer pathogenesis and different functional SNPs of genes involved in the main folate metabolism and the transsulfuration pathway. The current population-based, case-control study examined the association between MTHFD1 G1958A, MTHFD1 T401C, and the CBS 844ins68 insertion with breast cancer (BC) risk in Jordanian women. The studied population included 200  female BC subjects and age-matched female controls. The targeted genotypes MTHFD1 G1958A and MTHFD1 T401C were amplified via PCR followed by subsequent digestion with the proper restriction enzyme (PCR-RFLP), while the insertion/deletion of CBS844ins68bp was visualized and scored directly after gel electrophoresis. Results showed that the examined individual alleles and genotypes of MTHFD 1958A, MTHFD1 401C, and CBS844ins68bp per se were not associated with risk of BC compared with their wild-type genotypes and alleles.

Keywords: Breast cancer; CBS 844ins68; Folic acid; Jordan; MTHFD1 G1958A; MTHFD1 T401C

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