Assessment of Anticancer Activity of Crude Ethanolic Extracts of Moringa Oleifera Pod and Leaves on 7,12 - Dimethylbenz Anthracene Induced Skin Cancer in Mice.
S Saradha1
, R Abitha2, K Hari Prasath3, S Logithkumar4*, R Vijayashree5, T Sobita Devi 6 and P Indhra71Department of Pharmacology, Shri Sathya Sai Medical College and Research Institute (SSSMCRI), Sri Balaji Vidyapeeth (Deemed to be University), Ammapettai, Chengalpet district, Tamilnadu, India.
2Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chengalpet district, Tamil Nadu, India.
3Department of Pathology, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chengalpet district, Tamil Nadu, India.
4Central animal house, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chengalpet district, Tamil Nadu, India.
5Department of community medicine, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chengalpet district, Tamil Nadu, India.
Corresponding Author E-mail: logithkumars2001@gmail.com
Abstract: Background and Objective: Carcinoma of the skin is the commonest cancer in the world. This study aims to assess the anti-cancer effect of the ethanolic pod and leaf extracts of Moringa Oleifera on 7,12 - dimethylbenz anthracene (DMBA) induced skin carcinoma in mice. Methodology: Animals were divided into 6 groups of 5 each. 7,12 - dimethylbenz anthracene (DMBA) was used topically for four weeks to induce tumour. Group 1 received placebo, Group 2 - standard drug 5- Fluorouracil, Groups 3, 4 received pod extract and Groups 5,6 received leaf extract of Moringa Oleifera of concentration 500 and 1000mg/kg respectively for 3 weeks. Hematological and biochemical parameters such as Hemoglobin, RBC, WBC and platelet counts, SGOT and SGPT, blood urea nitrogen and serum creatinine were done before cancer induction and at the end of 7 weeks. Histopathological examination of the skin, liver and kidney were done at the end of 7 weeks. Results: There was reduction in tumor size in the Moringa Oleifera pod and leaf extract treated groups. Histopathology revealed infiltration of the cells with scarring of epidermis in the extract treated groups indicating the healing of tissues more pronounced at higher concentration. Control group showed atypical squamous cells whereas the standard drug treated group showed infiltration and scarring. Conclusion: This study exhibits a dose dependent anticancer effect of Moringa oleifera pod and leaf extracts in mice which was comparable with the standard drug 5-Fluorouracil.
Keywords: Anti-cancer effect; DMBA, 5-Fluorouracil; Moringa Oleifera; Pod and Leaf extracts; Skin cancer Back to TOC