Evaluation of Anti-epileptic Effect of Sinapis alba using Maximal Electroshock Seizure Model
Gayatri R Nayak1
, Shreya Hegde1, Smita Shenoy1, Amrita Parida1*, Mohandas Rao KG2, Shivakumar Reddy K3, Shoa Nayyer4 and Manju V51Department of Pharmacology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal Karnataka, India.
2Professor of Anatomy, Department of Basic Medical Sciences, Manipal, Manipal Academy of Higher Education, Manipal Karnataka, India.
3Centre for Molecular Neurosciences, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal Karnataka, India.
4Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal Karnataka, India.
5Department of Paediatrics, Dr. TMA Pai Rotary Hospital, Karkala, Manipal Academy of Higher Education, Manipal, Karnataka, India.
Corresponding Author E-mail:amrita_parida@yahoo.com
Abstract: Background: Epilepsy is a prevalent neurological disorder, prompting an ongoing quest for new therapeutic agents. Sinapis alba, commonly known as yellow mustard, has garnered interest for its potential medicinal properties. This study aimed to assess the anti-convulsant potential of Sinapis alba in rats. Methods: Wistar albino rats were categorized into 5 distinct experimental groups (six each) : a normal control, a disease control, a group administered Sinapis alba seed oil (200mg/kg body weight), another given sodium valproate (300mg/kg body weight), and a combination group receiving both Sinapis alba seed oil and sodium valproate (150mg/kg body weight each). Antioxidant markers were subsequently extracted from the brain samples, and cresyl violet staining was employed to discern pathological changes. Results: The findings revealed a significant diminution in the durations of flexion, clonic convulsion, and stupor in the test, standard, and combination groups in contrast with the disease control. Additionally, the duration of tonic hind limb extension (THLE) noted a substantial decrease in the Sinapis alba group, sodium valproate group, and combination group. Moreover, the administration of Sinapis alba seed oil led to an elevation in antioxidant levels and a concomitant reduction in lipid peroxide levels. Intriguingly, a synergistic effect on generalized tonic-clonic seizures was observed upon integrating mustard oil with sodium valproate. Conclusion: Our research suggests that Sinapis alba seed extract demonstrates promising anti-epileptic properties and stands as a potential supplementary drug for managing generalized tonic-clonic seizures.
Keywords: Epilepsy; Pharmacognosy; Plant Products; Seizures; Yellow Mustard Back to TOC