Whole Genome Sequence for Klebsiella pneumoniae isolate from Burn Skin

Namariq Al-Saadi¹, Ohood A. Radhi² and Ali Adnan Hashim¹

¹Al-Manara, College for Medical Sciences, Misan, Iraq.

²Department of Basic Science, College of Nursing, University of Kufa, Iraq.

Corresponding Author E-mail: nha15@scarletmail.rutgers.edu

Abstract: Klebsiella pneumoniae is a type of gram-negative bacterium that was initially discovered and isolated by Carl Friedlander in the year 1882. Klebsiella pneumoniae is responsible for a range of nosocomial and community-acquired illnesses, including urinary tract infections (UTIs), pneumonia, surgical site infections, and bloodstream infections. According to a number of studies, gram-negative sepsis ranks as the second most prevalent cause. A comprehensive analysis of the entire genome of Klebsiella pneumoniae was conducted using Illumina sequencing by synthesis (SBS) technology, specifically the Illumina HiSeq platform. The genetic material of the organism was comprised of a single circular chromosome of three thousand base pairs in length, exhibiting a GC content of 55.90%. In total, the genome exhibited a collective count of 164 genes associated with transfer RNA, 9 genes associated with ribosomal RNA, and 11,132 protein-coding sequences. Moreover, the analysis of genetic diversity entailed the application of sequence data, which was then compared to the reference genome that shared the highest degree of similarity. The genome annotation service offered by PATRIC and the RAST annotation system have successfully detected many antimicrobial resistance elements, clusters of genes associated with antibiotic resistance, as well as efflux pumps. This study represents the first known case of conducting a comprehensive genome sequencing of Klebsiella pneumoniae within the geographic area of Iraq, based on our current knowledge. The current investigation holds potential for advancing our comprehension of the antibiotic resistance traits demonstrated by Klebsiella pneumoniae, therefore enabling the healthcare facility to efficiently handle and alleviate outbreaks.

Keywords: Klebsiella Pneumoniae; Skin Burn; Whole Genome Sequencing

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