In-Vitro Assessment of Antioxidant and Antimicrobial Activity of Field Grown and Tissue Cultured Fruit Callus of Nothapodytes nimmoniana

Vanshika Shrivastava¹, Naveen Sharma^2*, Vikas Shrivastava¹ and Kuldeep Dwivedi³

¹Amity Institute of Biotechnology, Amity University Gwalior, Madhya Pradesh, India.

²Amity Institute of Pharmacy, Amity University Gwalior, Madhya Pradesh, India.

³Department of Environmental Science, Amity University, Gwalior, Madhya Pradesh, India.

Corresponding Author E-mail: naveenpcol@gmail.com

Abstract: This investigation aimed to evaluate in vitro antioxidant and antimicrobial activity from the methanolic extracts of tissue-cultured and field-grown parts of Nothopodytes nimmoniana. Antioxidant activity of the plant parts was measured by Di-phenyl-picryl-hydrazyl (DPPH) radical, reducing power, and hydrogen peroxide assays. Antimicrobial activity of plant extracts was evaluated by the well diffusion method and the MIC values determined against different pathogens such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, and Candida albicans. The DPPH assay showed overall strong antioxidant activities from the suspension culture and fruit callus culture with an IC50 value. Antimicrobial activity against 4 bacteria and 1 fungus was evaluated through the well-diffusion method and by determining the minimum inhibitory concentration (MIC). The suspension culture and fruit callus showed better antimicrobial activity than other plant parts. The zone of inhibition showed maximum values in suspension culture and fruit callus extract against Candida albicans. The study implies that suspension culture and fruit callus culture of plants showed the greatest values because of the enhancement of the alkaloid compound camptothecin and other compounds like phenolics and flavonoids.

Keywords: Antioxidant activity; Antimicrobial activity; Camptothecin; Nothapodytes nimmoniana

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