Simultaneous Estimation of Curcumin and Gentamicin by UV-VIS Spectrometric Methods or Derivative Spectroscopic Techniques
Monika Sharma1
, Diksha Gulati1, Anjoo Kamboj1 and Saahil Arora2*1Chandigarh University Gharuan, Chandigarh College of Pharmacy, CGC Campus, Landran, Mohali, Punjab, India.
2University Institute of Pharma Sciences, Chandigarh University, Chandigarh Ludhiana Highway, Mohali, Punjab India.
Corresponding Author E-mail: saahil70@gmail.com
Abstract: A simultaneous study is very important analytical parameter which helps to assess compatibility of mixture of drugs. UV spectrophotometer is one of the simplest and efficient methods to assess simultaneous parameters. Due to its economical approach, we have used UV spectrophotometer. Curcumin and Gentamicin sulphate have zero crossing points of 420 nm and 244 nm, respectively, in first-order derivative spectroscopy. The solvent for the spectrophotometric process was 0.1 N NaOH. Curcumin and Gentamicin sulphate linearity was established over range of concentrations of 2–12 g/ml, with correlation coefficients of 0.995 and 0.993. The mean percent recoveries Curcumin and Gentamicin sulphate were found to be in the range of 98.88 percent and 98.54 percent, respectively. The approach has been found to be repeatable in both inter day and intraday testing. The approach was proven to be both precise and reliable. According to the recovery investigation, the approach was effectively applied to pharmaceutical formulation with no interference from excipients. The results of the analysis were statistically evaluated, as well as by recovery trials. The LOD and LOQ for Gentamicin sulphate in phosphate buffer were found to be 0.024µg/ml and 0.045µg/ml, respectively and values for Curcumin were 0.024µg/ml and 0.037 µg/ml, respectively which indicates adequate sensitivity of method
Keywords: Curcumin; Burn Wound Infections; Gentamicin sulphate; Simultaneous equation method; Validation Back to TOC