Isolation and Evaluation of Cytotoxic, Anti-Inflammatory, Anti-Ulcer Activity of Methanolic Extract of Ceriops decandra leaves
Kamrun Nahar1
, Fatema-Tuz-Zohora2*, Rayhana Begum1, Maruf Hasan1 , Abdul Aziz1, Yasmin Jui1, Muhammad Abdullah Al-Mansur 3 and Md. Rafi Anwar41Department of Pharmacy, Primeasia University, Bangladesh. Star Tower, 12 Kemal Ataturk Ave, Dhaka.
2Department of Pharmacy, University of Asia Pacific, Bangladesh. 74/A Green Rd, Dhaka.
3Institute of National Analytical Research and Service (INARS), BCSIR; P9RP+375, Dr. Qudrat-E-Khuda Road Dhaka.
4University of Louisiana at Monroe, College of Pharmacy, Louisiana, USA.
Corresponding Author E-mail:fatema.zohora41@gmail.com
Abstract: The current study was undertaken to provide scientific validation for the traditional medicinal applications of Ceriops decandra leaves in treating gastrointestinal disorders and inflammation. Additionally, the study aimed to isolate a pure component from the extracted leaves for further analysis. Lupeol was extracted from the crude methanolic extract of Ceriops decandra leaves by column chromatography as part of a phytochemical inquiry. Its structure was determined using 1H and 13C NMR spectroscopy. In order to assess the cytotoxicity, the unrefined methanolic extract was divided into two fractions: a petroleum fraction and an aqueous fraction, employing the modified Kupchan method. The brine shrimp lethality test revealed that both the aqueous and petroleum ether fractions had significant cytotoxic activity, with LC50 values of 1.93 µg/l and 2.04 µg/l, respectively. These values were compared to the LC50 value of the standard Vincristine Sulphate, which was found to be 0.02 µg/l. The results of the anti-inflammatory trial demonstrated that the administration of the extract at doses of 250mg/kg and 500mg/kg resulted in protection rates of 62.5% and 87.5%, respectively, as compared to the carrageenan control group after 3 hours post-injection. It is worth noting that Ibuprofen exhibited a higher level of protection, with a rate of 91.7%. In the context of ethanol-induced stomach ulcer, the administration of extracts at doses of 250 mg/kg and 500 mg/kg resulted in 45.5% and 59.1% protection against gastric ulcer, respectively. These findings were compared to the protective effect of Omeprazole, which demonstrated 63.6% protection and served as the standard reference. The findings suggest that the methanolic leaf extract of Ceriops decandra possesses robust cytotoxic and potent anti-inflammatory and anti-ulcer properties. These results provide support for the traditional application of this extract in the management of gastrointestinal diseases, inflammation, and cancer.
Keywords: Anti-Inflammatory; Anti-Ulcer; Cytotoxicity; Isolation; LC50; NMR Back to TOC