HPTLC Method for Estimation of Metformin Hydrochloride
C. H. Madhusudan Reddy, G. Mubeen* and Mamta PalAl-Ameen College of Pharmacy, Bangalore - 560 027 (India).
Abstract: HPTLC method for estimation of metformin HCl in single and in combination dosage form was developed and validated by using mobile phase consisting of methanol: chloroform: ammonium acetate (6:3:1 v/v/v). Densitometric analysis of metformin hydrochloride was carried out in the absorbance mode at 236 nm.The linearity was found to be in the concentration range of 100- 300 ng/spot. The % recovery of metformin hydrochloride was found to be 100.24-101.58%, indicating no interference from the excipients in the method.
Keywords: HPTLC; Metformin HCl; validation Back to TOC