Phytochemical Profiling and Evaluation for Anti-oxidant, Thrombolytic, and Antimicrobial Activities of Moringa oleifera Lam Leaves Extracts
Khandokar Nabila Sultana Amee1, Zahirul Islam2
, Ferdousi Jahan Sumi3, Mohammad Ibrahim Mondol1, Mahfuza Afroz Soma4 , Md. Harun-Or-Rashid2 , Md. Iqbal Shahid5, Md. Hassan Kawsar1 , Md. Jamal Hossain1 , Raquibul Hasan6 , and Md. Moklesur Rahman Sarker1,5*1Department of Pharmacy, State University of Bangladesh, 77, Satmasjid Road, Dhanmondi, Dhaka 1205, Bangladesh.
2Department of Pharmacy, World University of Bangladesh, Dhaka 1205, Bangladesh.
3Department of Botany, Government BM College, Barishal 8200, Bangladesh.
4Department of Pharmacy, University of Asia Pacific, 74/A, Green Road, Dhaka 1205, Bangladesh.
5Pharmacology and Toxicology Research Division, Health Med Science Research Network, 3/1 Block F, Lalmatia, Dhaka 1207, Bangladesh
6Department of Pharmaceutical Sciences, College of Pharmacy, Mercer University, Atlanta, GA 30341, United States
Corresponding Author E-mail: moklesur2002@yahoo.com
Abstract: The present study was undertaken for phytochemical screening as well as evaluating in vitro antioxidant, thrombolytic, and antimicrobial properties of crude methanolic extracts of Moringa oleifera Lam leaf and its diversified extractives. The antioxidant potential was investigated by ascertaining the capability of the samples to DPPH scavenging with phenol compositions estimated. Besides, thrombolytic and antibacterial properties were assessed following clot lysis and disc diffusion methods, respectively. All fractions were found to contain flavonoids, reducing sugars, tannins, gums, saponins, and quinines, while ME, PESF and CTSF comprise glycosides, steroids, and terpenoids. In the antioxidant activity assay, CTSF possessed the uppermost phenolic content (34.38 mg of GAE/gm) and the scavengers of DPPH free radicals potential with IC50 value of 2.96 µg/mL associated to the standard drug ascorbic acid (2.48µg/mL). AQSF displayed the highest percentage of clot lysis (25.00%), compared to the conventional drug streptokinase (63.74%). The methanol extract, PESF and CSF of M. oleifera displayed antimicrobial activity against all tested microorganisms. Therefore, the outcomes of the existing study were accompanied by the validation of the antioxidant, thrombolytic, and antimicrobial properties of M. oleifera leaves, which justified the plant's use in traditional medicine.
Keywords: Antioxidant; Antimicrobial activities; Extract; Moringa oleifera Lam.; Phytochemicals; Thrombolytic Back to TOC