Effect of Wheatgrass Juice on Fertility Changes Induced by Cyclophosphamide in Male Wistar Albino Rats
Akshatha P Kamath1![](https://www.biomedpharmajournal.org/wp-content/uploads/2017/11/orcid_16x16.png)
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1Department of Pharmacology, Manipal College of Pharmaceutical sciences, Manipal Academy of Higher Education, Manipal, India
2Dean of Basic Sciences, All Saints University School of Medicine, Dominica.
3Department of Pharmacology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, India
4Department of Medicine, KS Hegde Medical Academy, Mangalore, India.
5Department of Department of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India
6Department of Pharmacology, All Saints University School of Medicine, Dominica
7Department of Physiology, AIIMS, Mangalgiri, Andhra Pradesh, India.
8Department of Paediatrics, Dr. TMA Pai Rotary hospital, Karkala, Manipal Academy of Higher Education, Manipal, India
9Kasturba medical college, Manipal, Manipal Academy of Higher Education, Manipal, India
10American University of Antigua, Antigua.
Corresponding Author E-mail: navin903@gmail.com
Abstract: In the present study, reproductive toxicity caused by cyclophosphamide on sperm parameters, biochemical parameters, histopathology and protective effect of wheatgrass juice was examined. There were total 6 groups of adult Wistar albino rats. Each group had 6 rats. After 24hrs of the last treatment, rats were sacrificed by cervical dislocation and sperm parameters like sperm count and motility, plasma malonaldehyde (MDA) levels, catalase (CAT) levels, superoxide dismutase (SOD) and glutathione (GSH) activities in testicular tissue, and testicular histopathological changes in the testicular tissue were examined. Administration of cyclophosphamide showed an increase in plasma MDA level and decrease in SOD, GSH and Catalase activity. These values were statistically significant. However, wheatgrass juice treatment along with cyclophosphamide markedly showed improvement in reducing Cyclophosphamide-induced oxidative stress, and normalized sperm characteristics and testicular histopathology. Thus, in conclusion, cyclophosphamide causes fertility changes which leads to male infertility, whereas wheatgrass juice has a protective effect on reversing the testicular damage and male infertility.
Keywords: Antifertility; Antioxidant; Sperm morphology; Testicular health Back to TOC