Effect of Wheatgrass Juice on Fertility Changes Induced by Cyclophosphamide in Male Wistar Albino Rats

Akshatha P Kamath¹ , Navin Patil^2*,  Amrita Parida³, Karthik Rao⁴, Nadeem Khan G⁵, Shanti Gurung⁶, Afreen Begum H Itagi⁷, Manju V⁸, Heraa Islam⁹, Aditi Kapoor⁹, Mohsin Nazeer⁹, Shreya Hegde³,  Nikhilesh Anand¹⁰

¹Department of Pharmacology, Manipal College of Pharmaceutical sciences, Manipal Academy of Higher Education, Manipal, India

²Dean of Basic Sciences, All Saints University School of Medicine, Dominica.

³Department of Pharmacology, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, India

⁴Department of Medicine, KS Hegde Medical Academy, Mangalore, India.

⁵Department of Department of Cell and Molecular Biology, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, India

⁶Department of Pharmacology, All Saints University School of Medicine, Dominica

⁷Department of Physiology, AIIMS, Mangalgiri, Andhra Pradesh, India.

⁸Department of Paediatrics, Dr. TMA Pai Rotary hospital, Karkala, Manipal Academy of Higher Education, Manipal, India

⁹Kasturba medical college, Manipal, Manipal Academy of Higher Education, Manipal, India

¹⁰American University of Antigua, Antigua.

Corresponding Author E-mail: navin903@gmail.com

Abstract: In the present study, reproductive toxicity caused by cyclophosphamide on sperm parameters, biochemical parameters, histopathology and protective effect of wheatgrass juice was examined. There were total 6 groups of adult Wistar albino rats. Each group had 6 rats. After 24hrs of the last treatment, rats were sacrificed by cervical dislocation and sperm parameters like sperm count and motility, plasma malonaldehyde (MDA) levels, catalase (CAT) levels, superoxide dismutase (SOD) and glutathione (GSH) activities in testicular tissue, and testicular histopathological changes in the testicular tissue were examined. Administration of cyclophosphamide showed an increase in plasma MDA level and decrease in SOD, GSH and Catalase activity. These values were statistically significant. However, wheatgrass juice treatment along with cyclophosphamide markedly showed improvement in reducing Cyclophosphamide-induced oxidative stress, and normalized sperm characteristics and testicular histopathology. Thus, in conclusion, cyclophosphamide causes fertility changes which leads to male infertility, whereas wheatgrass juice has a protective effect on reversing the testicular damage and male infertility.

Keywords: Antifertility; Antioxidant; Sperm morphology; Testicular health

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