Cytotoxicity Test of Active Compounds Natural Ingredients of Snail Mucus (Achatina fulica) Against BHK-21 Fibroblast Cells

Swastini IGAAP^1*, Ni Nengah Sumerti² and Ni Ketut Nuratni²

¹Department of Medical Laboratory Technology, Poltekkes Kemenkes Denpasar, Denpasar, Indonesia.

²Department of Dental Health, Poltekkes Kemenkes Denpasar, Denpasar, Indonesia.

Corresponding Author E-mail: agungswastini18@gmail.com

Abstract: Snails are unpleasant yet beneficial. Rural people have used one to treat illnesses like toothache for years. We will test snail's mucus Achatina fulica's cytotoxic activity against Baby Hamster Kidney (BHK-21) fibroblast cells at 12.5%, 25%, 50%, 100% and its resistance to Phorpyromonas gingivalis, Fusobacterium nucleatum, E. Faecalis, and S. aureus using Microtetrazolium (MTT) assay. The test and comparison solution was incubated with 5x103/100 l cells in 96-well plates. 5 mg/mL MTT completed the solution's incubation. ELISA readers measured purple color intensity. The formula transformed absorbance data at 595 nm into percent alive cells. ELISA readers read data. ANOVA, parametric Kolmogorov-Smirnov data normality test were performed. The cytotoxicity statistical test shows the following results:  12.5 % (0.76875 ±0.01117), 25% (0.49350 ±0.004796), 50% (0.30250 ±0.006658)   and 100% (0.171 ±0.10488). The lowest cytotoxicity of Achatina fulica snail mucus is 12.5% with an average of 0.768. Achatina fulica snail mucus resists Phorpyromonas gingivalis, Fusobacterium nucleatum, E. Faecalis, and S. aureus at 12.5%.

Keywords: Achatina fulica; BHK-21; cytotoxicity; fibroblast; periodontitis; Snail Mucus

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