In Vitro Microbial Efficacy Analysis of Tobracef, a Fixed Dose Combination of Ceftazidime and Tobramycin Against Acinetobacter lwoffii, Morganella Morganii, Enterobacter Cloacae, Hafnia Alvei, Citrobacter Freundii & Serratia Grimesii.
Sanjay Mohan Shrivastava*, Sanjeev Kumar Shukla and Manu Chaudhary.Venus Medicine Research Center, Hill Top Industrial Estate, Jharmajri EPIP, Phase I (Extn) Bhatoli Kalan, Baddi - 173205 (India).
Abstract: Ceftazidime belongs to cephalosporin class of antibiotics with broad spectrum activity. Tobramycin is an aminoglycoside antibiotic used to treat various types of bacterial infections, particularly gram negative infections. In present study is an attempt to determine efficacy of ceftazidime, tobramycin and Tobracef, their Fixed Dose Combination (FDC) on some microorganisms. Efficacy was evaluated on the basis of Minimum Inhibitory Concentration (MIC) and Antibiotic Susceptibility Test (AST). In Acinetobacter lwoffii, Morganella morganii, Enterobacter cloacae, Hafnia alvei, Citrobacter freundii and Serratia grimesii. MIC were found to be 8µg/l, 4µg/l, 16µg/l, 4 µg/l, 2µg/l and 1µg/l for ceftazidime respectively. In a tobramycin alone the MIC were found to be 4 µg/l, 8 µg/l, 8 µg/l, 8 µg/l, 4 µg/l and 8 µg/l respectively whereas in tobracef MIC were found to be 2 µg/l, 2 µg/l, 4 µg/l, 2 µg/l, 1 µg/l and 0.5 µg/l. Results of AST also showed more lytic zone by Tobracef in all organisms when compared with ceftazidime and tobramycin alone. These results indicate that the Tobracef has better bactericidal activity in comparison to ceftazidime and tobramycin alone in organisms under study.
Keywords: Minimum Inhibitory Concentration (MIC); AST; Ceftazidime; Tobramycin; Tobracef Back to TOC