Simultaneous Estimation of Ciprofloxacin and Tinidazole by U.V Spectrophotometer using a Hydrotropic Solubilization Technique

Km. Babita¹*, Kapil Kalra¹, Neetu Rajpoot¹, Poonam Joshi² and Vandana Pokhriyal¹

 ¹Alpine college of management and technology, Dehradun Uttarakhand India.

²Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun Uttarakhand India.

Corresponding Author E-mail: babitanegi495@gmail.com

Abstract: Advanced, easy and accurate UV spectroscopy technique (I & II) was developed for estimation of Ciprofloxacin and Tinidazole in bulk and tablet dosage form and validated using hydrotropic solubilization techniques. In Method I we focused on solving the simultaneous equation on the basis of absorbance measurement at 270nm and 320nm wavelength of Ciprofloxacin and Tinidazole individually in 2M urea solution which results in enhancement of solubility of CPH and TNZ to about 15 folds as compared to distilled water.Strategy II is lay out idea of Q-analysis in which absorbance was estimated at 288nm (iso-absorptive point) 270nm (λmax of CPH) in 2M urea. Ciprofloxacin and Tinidazole display linearity at chose frequencies and comply with Beer's regulation in the focus scope of 0.2-1.2μg/mL and 10 to 60μg/mL separately. Recovery reads up for CPS and TNZ were completed and rate recuperation of the medications gotten in the scope of 99.2 - 99.6% (Method A) and 98.0-100.8% (Method B) affirming the exactness of the proposed strategy. Both the techniques showed great reproducibility  and recuperation with %RSD less than 2. Factual approval of the information shows that the proposed strategies can be effectively applied for the standard examination of medications in business tablets.

Keywords: absorbance ratio method; hydrotropic agent; Simultaneous estimation method

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