Regenerative Properties of Polypropylene Mesh Coated with Thiotriazoline and L-aginine

S. O. Maslennikov¹, M. L. Golovakha¹, I. F. Belenichev² and L. V. Makyeyeva^3*

¹Department of Traumatology and Orthopedics, Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine

²Department of Pharmacology and Medical Recipes with the Course of Normal Physiology, Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine

³Department of Histology, Cytology and Embryology, Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine

Corresponding Author E-mail: lyudmylamakyeyeva@gmail.com

Abstract: Restoration and strengthening of soft tissue structures of the musculoskeletal system underlie the principles of regenerative medicine to improve treatment results and prevent possible complications. Influencing various parts of the regenerative process, it is possible to correct the recovery time, the morphological properties of the scar, and, as a result, the clinical results of treatment. We have proposed using a polypropylene mesh (PM) impregnated with a metabolitotropic solution to restore damaged and strengthen weakened structures. The work aimed to study the effect of PM with metabolite impregnation on the regeneration process of soft tissues. A series of morphological and biochemical studies were carried out on laboratory animals. The degree of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) mRNA expression was studied as indicators of tissue ischemia and their trophism in correlation with morphological changes in the zone of mesh implantation. Using a PM with a metabolitotrope increases the expression of eNOS mRNA by 10.8 times, and iNOS mRNA - reduces by 3.6 times. Morphologically coated PM causes a pronounced inflammatory effect, followed by a pronounced proliferation of connective tissue fibers around the mesh elements. Particular attention is drawn to the growth of the microvasculature and the increase in the number of anastomoses - 10.3% of the relative area compared to 8.8% in the group using PM without a metabolite. The metabolitotropic impregnation improves oxygenation and trophism of damaged soft tissues, which helps to stimulate regeneration.

Keywords: Nitric Oxide Synthase; Metabolites; Polypropylene mesh; Regeneration; Soft Tissue

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