Assessment of Specific and Non-specific Autoantibodies among Newly Diagnosed Type 1 Diabetes Mellitus Sudanese Patients
Hind Amin Ishaq1
, Mariam Abbas Ibrahim1, Amar Mohammed Ismail2, Nuha Eljaili Abubaker1 and Elyasa M Elfaki3*1Department of Clinical Chemistry, College of Medical Laboratory Science, Sudan University of Science and Technology, Sudan.
2Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, AL-Neelain University, Sudan.
3Department of Clinical Laboratory Sciences, College of Applied Medical Science, Jouf University, Al-Qurayyat, Saudi Arabia.
Corresponding Author E-mail: eelfaki@ju.edu.sa
Abstract: Background: Pathogenesis of type 1 diabetes mellitus is associated with the presence of specific autoantibodies and viral infection. Herein we aim to assess specific and nonspecific autoantibodies in newly diagnosed type 1 diabetes mellitus patients. Materials and Methods: In this case-control study 200 subjects were enrolled, classified into 100 newly diagnosed type 1 diabetes mellitus patients ages ranged from 1 to 16 years old, and 100 apparently health control age matched group. Serum anti-glutamic acid decarboxylase/tyrosine Phosphatase 2 (anti-GAD/IA2), anti-tissue transglutaminase (anti-tTG) and antinuclear antibodies were measured. Results: Of 100, 62(62%) were males, and 38(38%) were females. Anti-GAD/IA2, anti-tTG, and antinuclear antibodies were found to be significantly higher in the case than in the control group. Anti-GAD/IA2 and anti-tTG were associated with higher risk of type 1 diabetes mellitus (OR= 5.44, P= 0.000) and (OR=5.82, P= 0.009) respectively. Conclusion: Anti-GAD/IA2, anti-tTG and antinuclear antibodies are higher in type 1 diabetes mellitus patients. Moreover, anti-GAD/IA2 and anti-tTG are associated with a high risk of type 1 diabetes mellitus.
Keywords: ANAs; Anti-tTG; Anti-GAD/IA2; Celiac Disease; Type-1 DM Back to TOC