Phytochemical Screening and Bacterial Activity of Hylocereus polyrhizus Britton and Rose Peel against Staphylococcus epidermidis and Staphylococcus aureus
Nani Wijayanti DN*
, Liza Yudhistira and Ana Khusnul Faizah
Department of Pharmacy, Hang Tuah University, Surabaya, Indonesia.
Corresponding Author E-mail: nani.wijayanti@hangtuah.ac.id
Abstract: Hylocereus polyrhizus Britton and Rose fruit is a tropical plant that is popular and widely cultivated due to its qualities and advantages and high nutritional content. Some substances in Hylocereus polyrhizus Britton and Rose are plant sources rich in nutrients and minerals, namely vitamin B complex and vitamin C, protein, fat, carbohydrates, fiber, flavonoids, niacin, pyridoxine, cobalamin, phenolics, betacyanins, polyphenols, and carotenoids. This study aimed to assess the bacterial activity of the 96% ethanol extract of Hylocereus polyrhizus fruit peel against the growth of Staphylococcus epidermidis and Staphylococcus aureus using the well diffusion method. Maceration was used as an extraction method, and a phytochemical screening procedure was carried out according to the process from Harbone. The bacterial activity was conducted by the well diffusion method. Phytochemical screening yields from 96% ethanol extract of Hylocereus polyrhizus fruit peel showed the presence of alkaloids, steroids, flavonoids, terpenoids, tannins, polyphenols, and saponins. The findings of the bacterial activity test of 96% ethanol extract of Hylocereus polyrhizus fruit peel with a concentration of 20%, 60%, and 80% (b/v) indicated intense antibacterial activity against Staphylococcus epidermidis and Staphylococcus aureus.
Keywords: Antibacterial; Hylocereus polyrhizus fruit peel; Staphylococcus aureus; Staphylococcus epidermidis; Well diffusion Back to TOC