Cytotoxicity, Antidiabetic and Anticancer Studies of Insulin and Curcumin-Loaded Polymeric Nanoparticles

A.Asha^1* and G.S. Prabha Littis Malar²

Department of Chemistry and Research Centre, Scott Christian College (Autonomous), Nagercoil. Affiliated to Manonmaniam Sundaranar University, Abhishekapatti, Tiruneveli, Tamil Nadu, India.

Corresponding Author E-mail: ashaanbaianmsc@gmail.com

Abstract: Cytotoxicity measurement is needed for all drug-loaded nanoparticles. Because, if the nanoparticles have toxicity means, the drug-loaded polymeric nanoparticles cannot be used for the drug delivery. Generally cell viability is measured in the cytotoxicity measurement. In this work, the nanoparticle have synthesized from the natural polymeric material. These nanoparticles have been prepared using a nano-precipitation technique. Drugs, Insulin and Curcumin are added to these synthesized nanoparticles. This drug was coated on the surface of the nanoparticles to enhance the biocompatibility. These drug-loaded polymeric nanoparticles are used for the drug delivery. L929 cells have been to prove the cytotoxicity of these drug loaded polymeric nanoparticles by Neutral red assay method. From the cytotoxicity assay TPIG, TPCG and CCIG, CCCG nanoparticles are not cytotoxic. Insulin-loaded Tapioca/pectin and a Casein/chitosan nanoparticle were used to study the anti- diabetic assay. Curcumin-loaded Tapioca/pectin and Casein/Chitosan nanoparticle were used for Anti-cancer studies, by making use of Human Osteosarcoma cells (HOS). From these studies, the Insulin and Curcumin-loaded Tapioca/pectin and Casein/chitosan nanoparticles are not cytotoxic, and they can be used for drug delivery.

Keywords: Casein; Drug; L929 cells; Nanoparticles; Pectin; Tapioca

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