Antioxidant and Cardioprotective Activity of Indigofera barberi on Doxorubicin Induced Toxicity on Rats
Sk. Aminabee*
, K. Indraja, K. Matha Manogna, K. Naga Devika, K. Ramya Sri, A. Lakshmana RaoDepartment of Pharmacology, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Andhra Pradesh, India.
Corresponding Author E-mail: aminaammi786@gmail.com
Abstract: Background: Worldwide, the major death causing diseases are cardiovascular diseases and today the need for herb based therapeutics is needed. Present study was undertaken the whole plant of Indigofera Barberi (IB) to evaluate its cardioprotective activity against cardiotoxicity on rats induced by Doxorubicin (DXR). Methods: Soxlet extraction was used to prepare extracts. Preliminary phytochemical tests and in-vitro antioxidant activity are the methods used for standardization of all the extracts. Chloroform extract of Indigofera barberi (CEIB) and aqueous extract of Indigofera barberi (AQIB) are two extracts obtained from above activity were selected against induced cardiotoxicity of DXR to determine in-vivo cardioprotective activity. Total flavonoid and phenol content was determined. Endogenous antioxidants (MDA, GSH), ECG and histophological studies are the parameters of serum (CK, CK-MB, LDH) and non serum to evaluate the cardioprotective activity. Results: Serum elevated levels of biomarker, decreased antioxidant activity, changes in electrocardiogram (ECG) and histopathological studies are shown by DXR alone treated rats. The toxicity produced by DXR has reversed on the rats pre-treated with CEIB and AQIB. CEIB has shown more activity when compared to AQIB. Compared to standard vitamin E the activity of CEIB was found to be significant. Conclusion: The protective effect of IB plant on DXR induced cardiotoxicity was revealed. To understand the mechanism of action and to reveal phytochemical responsible for the said activity the further research to be undertaken.
Keywords: Antioxidant; Cardioprotective; Cardiotoxicity; doxorubicin; heart failure; Indigofera barberi Back to TOC