Thioredoxin1 Gene Modulates Bcl2/p53/NF-KB Signaling Pathways in Strawberry Extract/Paracetamol-treated Rat Model of Acute Liver Injury

Aysam Fayed¹, Hala O Ramadan¹, Soha A. Hassan², Mohammed A. Hussein^3* and Tamer Roshdy¹

¹Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Menoufia, Egypt.

²Basic Science Department, Faculty of Dentistry, October 6 University, Sixth of October City, Egypt.

³Biotechnology Department, Faculty of Applied Medical Health Sciences Technology, October 6 University, Sixth of October City, Egypt.

Corresponding Author E-mail: prof.husseinma@o6u.edu.eg

Abstract: When used in excess, the analgesic paracetamol can cause hepatic centrilobular necrosis, which can be fatal. The goal of this study was to see if strawberry extract could protect rats' livers from paracetamol-induced hepatotoxicity. Strawberry (75 and 150 mg/kg bw) and vit C (1 g /kg bw) were given orally, daily for 15 days demonstrated a significant reduction in the effects of caused changes in plasma cholesterol, triacylglycerol, phospholipids and vit C, TBARS, GSH, TNF-α, IL-4 and NO, AST, ALT, ALP, LDH, SOD, GPx and GSH levels. Furthermore, strawberry extract significantly inhibits hepatocyte B-cell lymphoma 2 (Bcl2) but significantly induces p53, NF-KB and Trx1 gene expression compared to paracetamol- treated rats. Histological examination showed that significant normalization has been observed in strawberry extract treated rats. Conclusions Strawberry extract shows considerable hepatoprotective benefits in the case of paracetamol-induced liver damage, confirming it's essential use as a treatment for liver damage.

Keywords: Hepatoprotective; Inflammatory Mediators; Oxidative Stress Biomarkers; Paracetamol; Strawberry

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