Influence of Physiological Donor of Nitric Oxide on Blood Serum Crystallostasis in Rats

Andrew K. Martusevich^1-,3*, Lida K. Kovaleva⁵, Konstantin A. Karuzin³, Alexandra V. Surovegina^1-3, Mikhail Yu. Artamonov¹

¹Laboratory of Translational Free Radical Biomedicine, Sechenov University, Moscow, Russia, 119991

²Laboratory of Medical Boiphysics, Privolzhsky Research Medical University, Nizhny Novgorod, Russia, 603005

³Bioniq Health-Tech Solutions Ltd., London, United Kingdom

⁴Department of Animal Physiology and Biochemistry, Nizhny Novgorod State Agricultural Academy, Nizhny Novgorod, Russia, 603117

⁵Department of Histology, Kuban State Medical University, Krasnodar, Russia, 350063

Corresponding Author E-mail: cryst-mart@yandex.ru

Abstract: The aim of the work was to study the effect of intraperitoneal administration of dinitrosyl iron complexes with glutathione ligands on the initiated crystallogenesis of rat blood serum. The experiment was performed on 70 male Wistar rats divided into 7 equal groups. The first group of animals was intact (without any manipulation). Rats included in the other groups were administered intraperitoneal injection of 1 ml. 0.9% sodium chloride solution daily for 10 days. At the same time, animals of the third to sixth groups were additionally added to the injected solution with dinitrosyl iron complexes with glutathione ligands (the concentration of the agent was 0.15; 0.30; 0.45 and 0.60 mM, respectively). The rats of the seventh group received 1 ml. an aqueous solution of glutathione (0.15 mM). The study of the effect of DNIC on the crystallogenic properties of rat blood serum was carried out by the method of comparative tezioraphy using a special system of criteria. The data obtained as a result of this study indicate the presence of modification of the crystallogenic (initiating) properties of rat blood serum during the course intraperitoneal administration of a physiological donor of nitric oxide to animals – DNIC, and this effect turns out to be dose-dependent and having an extremum in the region of 0.3-0.45 mM. Taking into account the fact that the crystallogenic properties are a reflection of the component composition and one of the physico-chemical parameters of the biological fluid, it can be assumed that the metabolic response of the body to the use of the compound is also dose-dependent. At the same time, relatively low doses of the agent have the most optimal effect on metabolic processes, stimulating the antioxidant potential of blood serum with a corresponding decrease in the intensity of lipid peroxidation processes, contributing to the optimization of energy metabolism and increasing the activity of detoxification enzyme systems. The results of the teziographic evaluation of animal blood serum samples after a course of injections of DNIC in various concentrations clearly indicate the activating effect of the compound with respect to the crystallogenic (initiating) properties of biological fluid, which is most pronounced when using the substance in 0.3- and 0.45-millimolar aqueous solutions.

Keywords: Blood Serum; Biocrystallomics; Crystallization; Dinitrosyl Iron Conplexes

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