In Vitro Antioxidant and Mast Cell Stabilizing Activity of Different Extracts of Vanda Spathulata Flowers

Prasad Konduri¹, Eswar Kumar Kilari² and Ravindra Babu Sajja^{1, 3*}

¹Department of Pharmacology, Shri Vishnu College of Pharmacy, Bhimavaram

 ²Department of Pharmacology , College of Pharmaceutical Sciences, Andhra University, Visakhapatnam.

³Department of Pharmacology, Malla Reddy Institute of Pharmaceutical Sciences, Secunderabad.

Corresponding Author E-mail: ravicology@gmail.com

Abstract: Objective: The objective of the present work is to evaluate the petroleum ether, ethyl acetate and methanolic extracts of vanda spathulata on experimental models for in vitro antioxidant activity and mast cell stabilizing activity. Methods: Mast cell stabilization effect was assessed using compound 48/80 induced mast cell degranulation in rat peritoneal mast cell. The antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Results and Discussion: Vanda spathulata methanolic extract (VSME) at doses of 200 and 400mg/kg exhibited significant protection (p<0.01) and petroleum ether (VSPE) and ethylacetate (VSEA) extracts at the dose of 400mg/kg showed significant protection (p<0.05) against mast cell degranulation. VSME revealed better DPPH radical scavenging activity (IC₅₀ value 38.39 µg/ml) which was closely resembled to standard ascorbic acid (IC₅₀ value 33.98 µg/ml) when compared to other extracts. Phytochemical study revealed the presence of alkaloids, saponins, flavonoids, tannins, steroids and glycosides. Conclusion: From the results Vanda spathulata shows mast cell stabilizing activity mainly due to phytochemical constituents and strong antioxidant property of plant extracts.

Keywords: Antioxidant; Comp 48/80; DPPH; Flavonoids; Mast cell degranulation; Vanda spathulata

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