Isolation and Identification of Listeria Monocytogenes in Bangalore City from Various Food Samples
Vedavati Goudar1, Kanthesh B M2 and Nagalambika Prasad1*

1Department of Microbiology, Faculty of Life Sciences, JSS Academy of Higher Education and Research, Mysuru.

2Division of Molecular Biology, Faculty of Life Sciences, JSS Academy of Higher Education and Research, Mysuru.

Corresponding E-mail: ambikap@jssuni.edu.in

Abstract: The current research emphasis on the isolation and differentiation of Listeria monocytogenes from different food samples most frequently infected with Listeriosis outbreaks. Crude chicken meat, raw milk, pasteurized cheese, ice cream and raw fish are samples from the city of Bangalore. The selective medium mainly used for the isolation of Listeria is oxford agar. Using isolated L. monocytogenes from food samples, morphologic and biochemical identification was carried out. 2 samples (fresh milk and Ice cream) were positive out of 5 samples; 3 samples (raw chicken meat, raw fish, and pasteurized cheese) were negative. The results conferred during this study indicate the contamination of Ice- cream and Raw Milk samples with L. monocytogenes.

Keywords: Contamination; Food samples; Food borne pathogen; Listeria Monocytogenes; Listeriosis

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