Antibacterial Activity 96% Ethanol Extract of Brown Seaweed (Padina australis) from Poteran Island Madura against Staphylococcus aureus ATCC 25923
Nani Wijayanti DN*
, Giftania Wardani Sudjarwo and Oki Nugraha PutraDepartment of Pharmacy, Hang Tuah University, Surabaya, Indonesia.
Corresponding Author E-mail: nani.wijayanti@hangtuah.ac.id
Abstract:
Brown seaweed is one of the natural resources of the sea which is very abundant and grows naturally on the coast of Indonesia, especially in Madura. Lately, disease due to infection is one of the problems in the health sector that continues to grow. Some microorganisms that cause infections include bacteria, viruses, fungi and protozoa. One of the bacteria that can cause infection is Staphylococcus aureus. Staphylococcus aureus is one of pathogenic bacteria that cause abnormalities and infections to skin. The more infections cause an increase in the use of antibiotics, the greater the risk of increasing the incidence of resistance. This encourages the research of sources for antibacterial medicines from natural materials, namely macro algae. One of the macro algae that have antibacterial activity is brown seaweed from Padina australis species. This study uses laboratory experimental methods aimed at proving the antibacterial activity of 96% ethanol extract of brown seaweed Padina australis from the Madura Islands against Staphylococcus aureus ATCC 25923. The antibacterial activity test was carried out by the well diffusion method. The results of the antibacterial activity test showed that ethanol extract of 96% brown seaweed Padina australis with concentrations of 20%, 15%, 10%, and 5% had antibacterial activity against Staphylococcus aureus ATCC 25923 in the medium to strong category. Statistical test results (Kruskal Wallis) showed that there was a significant difference between the concentration and the inhibited zone produced (p = 0.037).
Keywords: Antibacterial; Ethanol 96% Extract; Padina australis; maceration; Â Staphylococcus aureus; Well Diffusion Method Back to TOC