Investigating the Effect of Borago Officnale on Hipocampal IL-1 Beta Protein and Gene in the Amyloid Β-Peptide (25–35)-Induced of Inflammation in Rat

Ehsan Barati^*1, Sara Soleimani Asl², Seyed Ali Pourbakhsh³, Mahmoud Jamshidian³ and Siamak Shahidi⁴

¹Student of Veterinary Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran ²Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran ³Department of Microbiology ,Science and Research Branch, Islamic Azad University, Tehran, Iran ⁴Department of Physiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Abstract: Alzheimer’s disease (AD) is a neurodegenerative disorder and most common form of dementia that leads to memory impairment. In the present study we have examined the investigating effect of Borago officinale on hipocampla IL-1 Beta protein in the Beta Amyloid peptid induced of inflammation in the rat. Wistar male rats received intrahippocampal (IHP) injection of the Aβ(25–35) and borage extract throughout gestation (100 mg/kg).inflamation was confitmed by pathologist.for mesearing the expression of the IL-1 Beta gene we used RT- PCR method and for extraction of the IL-1 Beta protein we used western bloting method.result showed that using Borago officinale lead to reduce IL-1 Beta gene and protein. This data suggests that borage could improve the reduction of inflammation factors such as IL-1 Beta and borage consumption may lead to an improvement of AD-induced cognitive dysfunction.

Keywords: IL1-Beta; Alzheimer; RT-PCR; Western Blot; Amyloid β-Peptide

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