Ethanol Extract of Young Papaya Seeds (Carica Papaya, L ) Lower the Level of testosterone, Spermatozoa Count and Expression of Androgen Receptors in Sertoli Cells of Adult Mice (Mus Musculus)
I Dewa Made Ruspawan1, I Made Bakta2, I Nyoman Mangku Karmaya2, Bagus Komang Satriyasa2,3 and I Gusti Kamasan Nyoman Arijana41Department of Nursing, Health Ministry of Polytechnic, Bali-Indonesia
2Doctoral Program of Medicine, Faculty of Medicine, Udayana University, Bali-Indonesia.
3Department of Pharmacology and Therapy, Faculty of Medicine, Udayana University, Bali-Indonesia
4Histology Department, Faculty of Medicine, Udayana University, Bali, Indonesia
Corresponding Author Email: ruspawan.dm@gmail.comAbstract: Background: Ethanol extract of young papaya seeds (Carica papaya L.) contains steroids that thought to has the antifertility property. This study aims to prove this hypothesis by measuring the low levels of testosterone hormone, spermatozoa count, and androgen receptor expression in sertoli cells. Material and Method:We use the randomized post-test only control group design on thirty-six male Balb/c strain adult male mice aged 12-14 weeks, weight 20-25 grams. They were randomly divided into a control and treatment group. The treatment group was given the same food as the control group plus ethanol extract of young papaya seeds 20 mg/20 gram body weight as much as 0.5 ml, orally every day for thirty-six days. Data were analyzed using the independent-sample T-test and Mann Whitney test. Result:The levels in the treatment group were significantly (p<0.05) lower than those in the control. Testosterone levels 31,64±1,91 vs 48,67±1,81 nmol/L, spermatozoa count 42,72±3,33 vs 75,89±4,71 cell/field of view, and androgen receptor expression 28,11±3.06% vs 55.07±2.49%. Conclusion:The ethanol extract of young papaya seeds 20 mg/20 g body weight has the antifertility property.
Keywords: Androgen Receptors; Ethanol Extract Of Young Papaya Seeds; Sertoli Cells; Spermatozoa; Testosterone Hormone Back to TOC