Evaluation of Homocystein and Micro RNA as Diagnostic Markers for Hepatocellular Carcinoma in Virus Hepatitis C Egyptian Patients
Eman A . Elghoroury1, Esmat E. Abdelghaffar1, Aliaa Ahmed wahby1, Soha A Nasr1, Mohamed A. Hussein2, Walaa S. Nazim3, Gamila S.M. El-Saeed4, Eman Refaat Youness4* amd Hisham A. Orban41Clinical Pathology Department, National Research Centre Cairo Egypt.
2Internal Medicine Department, Cairo University, Egypt.
3Genetic Biochemistry Department, National Research Centre, Cairo Egypt.
4Medical Biochemistry Department, National Research Centre. Cairo Egypt.
Corresponding Author E-mail: hoctober2000@yahoo.com
Abstract: In Egypt the incidence of HCC is increasing because of the high incidence of HCV infection. HCC starts silently with mild clinical and pathological deviation from chronic liver disease. Detection of specific and sensitive marker to help early prediction of such deviation to afford proper treatment was our aim.120 subjects were enrolled in the study, 40 patients suffering from chronic viral hepatitis C,40 developed hepatocellular carcinoma after HCV chronic hepatitis and 40 healthy controls. Liver functions,α-Fetoproteins (αFP), homocysteine(Hcy). Heat shock protein 70 (HSP 70) were done for all the subjects, MicroRNAsRQ26a and RQ27a were done using the RT-PCR method. Our results showed a significant difference between each of HCV & HCC group and the controls (P<0.05), while the difference between HCC and HCV group was highly significant (P<0.001) Except HSP70. RQ26a and RQ27a were down regulated in HCC group when compared to HCV group. It was previously shown that there was an inverse correlation between (RQ26a and RQ27a) results and each of, α FP, homocysteine and HSP70 within the HCC group. Again the same inverse correlation exists with Hcy. In conclusion, MicroRNAs (RQ26a and RQ27a) group, either solely or in combination with homocysteine might actually be used to classify sections with respect to progression to HCC and liver function, helping to reach treatment protocols and reside prognostic criteria.
Keywords: α-Fetoprotein; Hepatitis; Hepatocellular carcinoma; Homocysteine; MicroRNAs; Viral hepatitis. Back to TOC