Protective Effect of Resveratrol on REM Sleep Deprivation Induced Changes in Testicular Functions in Wistar Rats
R. V. Rohit Vishwanath1, S. Saradha2*
, A. Ruckmani2, A. Sindhana1, R. Vijayashree2, T. Sobita Devi31Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai-603103, Tamil Nadu, India.
2Department of Pharmacology, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai-603103, Tamil Nadu, India
3Department of Pathology, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakam, Chennai-603103, Tamilnadu, India.
3Central Animal House, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, Chennai-603103, Tamil Nadu, India.
Corresponding Author E-mail : saradhachoks@gmail.com
Abstract: Objective: To evaluate the effect of Resveratrol on testicular functions in rapid eye movement (REM) sleep deprived Wistar rats. Method: Eighteen male Wistar albino rats were divided into three groups of 6 rats each. Group1,(Control) had rats with normal sleep/wake cycle. Group 2 and 3 rats were subjected to seven days of REM sleep deprivation. Group 3 in addition received 20 mg/kg/day of Resveratrol. REM sleep deprivation was done using the inverted flower pot technique. Sperm count and motility, testosterone level, and histo pathology of testes was assessed among the three groups. One way ANOVA was used to assess the difference among the groups. p value<0.05 was considered statistically significant. Results: Sperm count and motility as well as testosterone level were within normal range in group 1. Group 2 had significant reduction in sperm count, motility and testosterone level (p<0.05). Group 3, treated with resveratrol in addition to REM sleep deprivation, showed improved sperm count, motility and testosterone and these levels were equivalent to group 1.The difference between group 2 and group 3 was found to be statistically significant(p <0.05). Histopathological analysis of the testes revealed normal architecture in group 1 and mild spermatogenic disarray with distorted architecture in group 2. However in group 3 no significant change was observed. Conclusion: Resveratrol was found to protect the testis from the damage due to REM sleep deprivation in rats in terms of sperm count, motility, testosterone levels and testicular morphology.
Keywords: Antioxidants; Infertility; REM Sleep Deprivation; Resveratrol; Sperm; Testes Back to TOC