Characterization and Identification of Methicillin-resistant Staphylococcus aureus (MRSA) Producing Biofilm: Impacts of Garlic Extract and Lactobacillus Biosurfactants
Essam Hassan Mohamed1,2*
, Salama Mostafa Abdel-Hafez1,3, Mohamed Mohamed Soliman4,5, Saad H. Alotaibi6, Adel Alkhedaide4 and Mostafa Abdulatif Mostafa1,71Department of Medical Microbiology, Faculty of Applied Medical Sciences, Taif University, Turrabah, 21995, Saudi Arabia.
2Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
3Animal Reproduction Research Institute, Immunobiology and Immunopharmacology Unit, Giza, Egypt.
4Clinical Laboratory Sciences Department, Turabah University College, Taif University, Turabah, 29541, Saudi Arabia.
5Biochemistry Department, Faculty of Veterinary Medicine, Benha University, Benha 13736, Egypt.
6Chemistry Department, Turabah University College, Turabah 29541, Taif University, Saudi Arabia.
7Zoology and Nematology Department, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt
Corresponding Author E-mail : esam2005micro@gmail.com
Abstract: The current study was intended to characterize and specify local MRSA isolates based on its phenotypic and genotypic features. Moreover, the anti-biofilm impact of garlic and lactobacillus biosurfactants against S. aureus was examined. A total of 130 samples and isolates were taken from four sheep farms. Ninety samples were nasal, 10 were lung tissues and the remaining 30 were raw sheep meat from local grocery stores. All isolates were examined for the presence of S. aureus by routine and known microbiological tests. Oxacillin and cefoxitin disc diffusion test were done and findings were confirmed by PCR technology for S. aureus specific genes. Lastly, garlic water extract (gWE) and Lactobacillus biosurfactants were examined for their anti-biofilm activity utilizing a microtitre plate test. Fifteen isolates out of 75 S. aureus were specified MRSA. Concerning the PCR results, the 16S rRNA, the nuc and the mecA genes were detected in 75, 15 and 15 isolates, respectively. Moreover, nuc gene was present in all MRSA isolates (100%). The biofilms formation by S. aureus MRSA was repressed significantly by garlic water extract (gWE) and was dose dependently effect. Same repression was reported by Lactobacilli biosurfactants in dose dependent manner. The prevalence of S. aureus MRSA was confirmed and S. aureus biofilm activity was repressed by gWE and Lactobacilli biosurfactants.
Keywords: Anti-biofilm Activity; Adhesion Molecules; Congo Red; Garlic Extract; PCR; Staph MRSA Back to TOC