In-Vitro Antimicrobial and Antioxidant Activity of Securinega leucopyrus (Willd) Muell
Dudhamal Tukaram Sambhaji1
, Ajmeer Ahmad Shahan2 and Vijay Kumar31Department of Shalyatantra, Institute for Post graduate Teaching and Research in Ayurveda (I.P.G.T. and R.A.) Gujarat Ayurved University, Jamnagar, Gujarat-361 008, India.
2Maharshi Hospital, Mount Lavinia, Colombo, Sri Lanka
3SRF all India Institute of Ayurveda, New Delhi, India
Corresponding Author E-mail : drtsdudhamal@gmail.com
Abstract: To evaluate antimicrobial and antioxidant activity of leaf and bark of Securinega leucopyrus for anti-microbial activity against clinically important bacterial and fungal strains using agar disc diffusion method and for antioxidant activity. The methanolic extracts of leaf and stem bark were tested for antimicrobial activity against four gram positive bacteria, four gram negative bacteria, antifungal activity and for their antioxidant activity using total antioxidant capacity, total reducing power, ferric reducing antioxidant power and DPPH free radical scavenging activity. Securinega leucopyrus (Willd) Muell. is traditionally used for the management of boils and wounds in Sri Lanka and India. This shrub is clinically evaluated for wound healing as case reports and some clinical trials also showed encouraging results in diabetic wounds. So it is need of an hour to evaluate its antimicrobial as well as antioxidant activity for scientific validation. The highest antimicrobial activity was exhibited by 250µg/ml methanolic extracts of both leaf and bark. The extracts at the 250µg/ml concentration shows maximum antifungal activity against Aspergillus flavus used in the study. Methanolic extracts of leaves exhibited highest radicals scavenging activity, that is, 88.14 percent. Similar results were observed in total antioxidant capacity, total reducing power and ferric reducing assay determination. Methanolic extract of leaf and stem bark at different concentrations showed in-vitro antimicrobial and antioxidant activity.
Keywords: Anti-Microbial; Antioxidant; Free Radical Scavenging; Humarai; Katupila; Securinega leucopyrus Back to TOC