Targeting the Extracellular Polysaccharide Production (EPS) by Biofilm Forming Bacteria from Orthodontic Brackets and Wires Through Antiquorum Sensing Action of Bioactive Compounds from Curcuma longa and Zingiber officinale
Sumathi C Samiappan1*
, Rajesh Pandiyan2, Sampathkumar Palanisamy1, Sivakumar Ramalingam1, Renuka Saravanan1, Sheik Abdulla Hameed11Department of Chemistry and Biosciences, Srinivasa Ramanujan Centre, SASTRA University, Kumbakonam, Tamil Nadu, India. 612001
2Department of Biochemistry, Karpagam University, Coimbatore, Tamil Nadu, India. 641021
Corresponding Author E-mail : sumathi.cs@src.sastra.edu
Abstract: The present study intends to study the biofilm forming indwellers of dental braces. Following standard pure culture techniques, twenty-eight strains were isolated and purified. Mostly the isolated colonies were Gram positive cocci and gram negative rod shaped bacteria. In mannitol salt agar (MSA) the golden yellow colour colonies were observed. The six isolates with yellow colour colonies confirms Staphylococcus aureus. Six isolates produced green colour colonies on Cetrimide agar confirmed as Pseudomonas aeruginosa. On blood agar supplemented with 5% sheep blood, nine isolates showed β -hemolysis, three isolates showed α-hemolysis and other isolates showed no hemolysis. All isolates tested were non lactose fermentors. Then the isolates were screened for the biofilm formation and were confirmed by Congo red agar method. Some of the isolates showed black colonies with a dry crystalline consistency which indicated positive for biofilm formation. The biofilm formation was also evaluated qualitatively by tube assay. Totally four isolates produced strong biofilms, six isolates produced moderate biofilm, seven isolates formed weak biofilms. Four isolates with strong biofilm formation chosen for further work. In antibiotic sensitivity test to observe the zone formation. Antibiotic resistance of biofilm forming bacteria was tested. The ethanol extracts of turmeric were separated by thin layer chromatography. Curcumin found as more effective compound when compared to the demethoxy curcumin and bis demethoxy curcumin against biofilm forming bacteria. Similarly, dry ginger extracts were also effective against biofilm forming bacteria. Ginger had more effective inhibition of biofilm formation than turmeric fractions.
Keywords: Antibiotic Resistance; Biofilm Forming Bacteria; Curcumin; Dental Braces; Dry Ginger; Orthodontic Wires Back to TOC