Cloning of Recombinant Human IL-29 (rhIL-29) from PBMC in Escherichia Coli.
J.Venkateshwara Rao
Department of Zoology, University College of Science, Osmania University, Hyderabad, Telangana – 500007, India
Corresponding Author E-mail : venbio@gmail.com
Abstract: In the present study Human Interleukin IL-29 was produced from human PBMC Cells (Peripheral Blood Mono Nuclear Cells). Upon induction with poly I:C, dendrocytes and PBMC cells produced IL-29. IL-29 molecule is well known to exhibit many anti-proliferative and anti-viral activities in cells and employed as therapeutic agent in many diseases. As its current production is very limited and insufficient, here in this study an attempted to produce it from PBMC cells and expressed in E.coli host. Plasmid vectors were designed with pET-3a vector series with restriction sites NdeI and BamHI and incorporated with IL-29 gene. In the current study the gene was successfully isolated from PBMC cells, m-RNA was extracted and purified converted in to c-DNA by RT-PCR and expressed in host cells. The expressed gene was confirmed on Agarose Gel Electrophoresis and sequences was confirmed with standard gene at NCBI. The study will help for further enhancement and maximise gene expression of IL-29 in host cells and also to scale upto higher levels for industrial scale productions.
Keywords: Antiviral; Anti-Proliferative; Cytokines; Interleukins; PBMC; rhIL-29 Back to TOC