Estimation of Load of Herpes Viruses in GCF and its Impact on Clinical Parameters in Periodontitis
S. Parthiban1, T. Ramakrishnan2, V. Shankarram3, V. Balakumar4, Rathika rai5, Umasudhakar61Senior lecturer, Department of periodontics., Thai moogambigai dental college, Golden George nagar, Chennai 600107 2Professor, Department of periodontics.,Adhi parasakthi dental college, Melmaruvathur, Tamil nadu 3Professor, Department of Periodontics., Thai moogambigai dental college, Golden George nagar, Chennai 600107 4Professor, Department of prosthodontics., Satyabama dental college, Chennai 5Professor, Department of prosthodontics., Thai moogambigai dental college, Golden George nagar,Chennai 600107 6Professor, Department of Periodontics., Thai moogambigai dental college, Golden George nagar, Chennai 600107.
Abstract: 1) To compare qualitative and quantitative profile of herpes viruses In gingival cervicular fluid of periodontitis and healthy periodontal tissues ; 2) To quantitatively assess the relation between HSV and periodontitis 40 patients with periodontitis(P) ,40 patients with gingivitis (G)and 40 with Healthy gingival tissues (HP) were recruited. GCF samples from periodontal sulcus were analyzed using quantitative real-time PCR to detect and quantify HSV virus. Frequencies of detection and levels of HSV were compared between healthy and diseased. Correlation between HSV viruses and Clinical parameters was assessed. Among the HSV viruses ,EBV prevalence was slightly higher in periodontitis sites compared with controls (33.3% vs. 23.8%; p>0.05); HSV-1 and HCMV viruses were less dominant.Significant correlation was seen between the HSV and periodontitis clinical parameters. Prevelance of HSV viruses in GCF and its correlation between the clinical parameters may represent an unspecific indicator for the host response in periodontitis.
Keywords: HSV viruses; GCF; Periodontitis Back to TOC