Comparative Inotropic Effects of the Some Isoquinoline Alkaloids

Inoyat Jumayev^1*, Pulat Usmanov¹, Shavkat Rustamov¹ and Sherzod Zhurakulov²

¹Institute of Biophysics and Biochemistry, National University of Uzbekistan, Tashkent, Uzbekistan.

²Institute of Chemistry of Plant Substances, Uzbek Academy of Sciences, Tashkent, Uzbekistan.

Corresponding Author E-mail : inoyat8585@mail.ru

Abstract: In this study, mechanisms of inotropic action of some isoquinoline alkaloids 1-(4-dimethylaminophenyl)-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline (F-24), 1-(2-chloro-4,5-methylenedioxyphenyl)-2-hydroxyethyl-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline (N-14) and 1-(2-сhloro-4,5-methylenedioxyphenyl)-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline (F-14) were studied. The F-24, F-14, and N-14 alkaloids have been shown to have a negative effect on papillary muscle contraction activity, IC₅₀ value -16,8 µM, 14,03 µM and 12 µM. Са²⁺_L-channel blocker - nifedipine, adenylate cyclase (AС) activator–forskolin, β-adrenoreceptor (β-AR) blocker – propranolol, protein kinase С (PKС) activator – phorbol 12-myristate 13-acetate and SR RyR2 activator caffeine were used. Inotropic effects of F-24, F-14 and N-14 isoquinoline alkaloids on cardiomyocytes were suggested, based on results obtained in experiments carried in cardiomyocytes β-AR → [cAMP] → PKA → [Са²⁺]_in ↑ cascade, PKC, RyR2 and Na⁺/Ca²⁺ modulation.

Keywords: Inotropic Effect; Isoquinoline Alkaloids; Papillary Muscle

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