The Effect of Sodium Tetraborate on Chromium-Induced Oxidative Damages in Rats Lung Tissue
Marat Iztleuov1
, Abdiraman Kaliev2, Aigul Turganbaeva1, Dariga Yesmukhanova1, Ainur Akhmetova1, Assem Temirbayeva1, Yerbolat Iztleuov3* and Gulmira Iztleuova4
1Department of Natural Sciences, Faculty of Medicine, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
2Department of Pathological Anatomy and Forensic Medicine, Faculty of Medicine, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
3Department of Radiation diagnostics, Faculty of Medicine, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
4Course of dermatovenerology, Faculty of Medicine, West Kazakhstan Marat Ospanov Medical University, Aktobe, Kazakhstan
Corresponding Author E-mail : ermar80@mail.ru
Abstract: The purpose of this research is to study the effect of sodium tetraborate on chromium-induced oxidative damage in lung tissue. The experiment was conducted on 60 «Wistar» male rats (170-190g.) which were divided into 6 groups. The first group is control; the second, third, fourth groups received potassium dichromate (K2Cr2O7) with drinking water at a dose of 700 mg/l for 21 days; the fifth and sixth groups of animals received orally a solution of sodium tetraborate (Na2B4O7) at a dose of 22.5 mg/kg and 225 mg/kg of body weight per day for 31 days. Animals of the third and fourth groups received orally Na2B4O7 at doses of 22.5 mg/kg and 225 mg/kg per day, respectively, for ten days before receiving K2Cr2O7, then for 21 days, co-administration of potassium dichromate. Introduction of K2Cr2O7 caused a change in the endopulmonary cytogram, significantly reduced the nonspecific mechanism of respiratory protection from external influences, increased the content of malonic dialdehyde (p<0.05) and same as previous. Co-administration of Na2B4O7 (22.5 mg/kg) with K2Cr2O7 improves the mucociliary index (anti-inflammatory effect - the effect of debridement), inhibits oxidative damage (oxidative stress) in the lung tissue (antioxidant effect) in comparison with the data of animals exposed to K2Cr2O7. The combined use of Na2B4O7 in a high dose with K2Cr2O7 did not show the expected positive effect. Oxidative stress in the lung tissue induced by K2Cr2O7 leads to changes in the endopulmonary cytogram, disturbances in the mucociliary index, the balance of prooxidants/antioxidants in the lung tissue, to an increase in the peroxidation of lipids and inhibition of the antioxidant system. Sodium tetraborate, depending on the dose (at a low dose of 22.5 mg/kg body weight), can protect lung tissue from chromium-induced oxidative damage. However, with the introduction of sodium tetraborate in a high dose with chromium, the expected positive effect is not observed.
Keywords: Chromium-Induced Oxidative Damage; Potassium Dichromate; Respiratory Protection; Sodium Tetraborate Back to TOC