Regulation of Sex Steroid Sex Hormones on Calcitonin Gene-Related Peptide (CGRP)’S MRNA Expression in Vaginal Mucosa Epitel of Bilateral Ovarectomized Wistar Rats
Luh Yenny Armayanti*
and Nadya Treesna WulansariInstitute of Technology and Health Bali, Jl. Tukad Balian No.180 Denpasar, Indonesia
Corresponding Author E-mail: armayanti61@gmail.com
Abstract: During menopause, there is a decrease in the level of synthesis of sex steroid hormones such as estrogen, progesterone and androgen. Dramatic changes in sex steroid hormone levels cause vulvovaginal atrophy which is characterized by vaginal dryness, atrophy, dyspareunia, and sexual arousal disorders. This mechanism is thought to be mediated by changes in the expression of calcitonin gene related peptide (CGRP), a neuropeptide which is a potent vasodilator. This study aims to determine how sex steroid hormones regulate the expression of Calcitonin Gene-RealtPeptide (CGRP) mRNA in the vaginal mucosal epithelium of wistar rats with bilateral ovarectomy. A post-test only control group design research was conducted on 30 female rats. Menopausal state was induced by ovarectomy on bilateral ovaries. After two weeks the rats were divided into 3 groups: the control group (given 1 ml aquadest), the treatment group I (given a combination of estrogen (11 μg / day) + progesterone (180 μg / day) and the treatment group II were given a combination of estrogen (11 μg / day) + progesterone (180 μg / day) + testosterone (360 μg / day) . After 30 days the mice were terminated and the vaginal tissue was taken from 2/3 part of posterior vagina. CGRP’s mRNA expression was calculated using the real time Polymerase Chain Reaction (rtPCR) method. The average CGRP gene mRNA levels in the control, treatment I and treatment II group were 1.60 ± 0.24 fg / ul, 4.77 ± 1.39 fg /ul, 7.09 ± 1.63 fg /ul respectively. There were significant differences in the mean mRNA levels of the CGRP gene between each group. This result suggests that the administration of sex steroid hormones increase CGRP mRNA expression.
Keywords: CGRP; mRNA; Ovarectomized Rats; Sex Steroid Hormone; Vaginal Mucosa Epithelium Back to TOC