Augmentation of Immunocytes Functions by Prunus Cerasus Fruit and its Biotherapeutic Potential in Mice Model
Sheikh Abid Ali1*, Refaz Ahmad1, Nazir Ahmad2, Mudasir Makhdoomi3 and Qazi Parvaiz11Division of Biotechnology, CSIR- Indian Institute of Integrative Medicine, Srinagar-190005, (J and K) India
2Department of Microbiology and Biotechnology, Bangalore University, Bangalore, Karnataka- 560056. India
3Department of Endocrinology, SKIMS- Srinagar, 190011, Kashmir (J and K) India
Corresponding Author E-mail: abidalis2007@gmail.com
Abstract: A substantial and growing body of scientific research has linked Sour cherries to various biotherapeutic properties and suggested as a candidate for immunomodulation. The effects of graded doses of a chemically standardized methanolic fruit extract (PcMFE) of Prunus cerasus on the immune system and anti-oxidative status of SRBC immunized BALB/c mice were investigated. Oral administration of PcMFE (100-250 mg/kg) enhanced the expression pattern of IgM and IgG titres, stimulated cell mediated immunity reaching peak value with 200 mg/kg b. wt. Flowcytometric analysis of surface markers of T cells (CD4+ and CD8+) and B cells (CD 19+) indicated prominent enhancement in proliferation and differentiation of these lymphocytes. The extract enhanced expression of T helper cells Th1 cytokines interferon (IFN)-γ and interleukin-12 (IL-12) in the sera of treated mice compared with the control group. In vivo studies showed PcMFE increased spleen and thymus indices, activated macrophage functions ex-vivo as indicated by nitroblue tetrazolium reduction potential, inducible nitric oxide synthase activity and bactericidal property significantly. Furthermore, the oxidative stress marker studies revealed that the administration of PcMFE significantly decreased levels of LPO, increased the activities of SOD, CAT and GSH-Px as compared to the control group. These findings indicate PcMFE has immunomodulatory activity in vivo and might play an important role in prevention of oxidative damage in immunological system.
Keywords: CAT; Cytokines; Delayed Type Hypersensitivity; Haemagglutination Antibody Titre; HPLC; iNOS; Levamisole, MDA; NBT; Phagocytosis; SOD Back to TOC