A Comparative Study of the Effect of Lignocaine and Tramadol on Healing of Infected wound in Wistar Rats
Smita Shenoy1
, Abhijna Ballal R2*, Praveen Kumar S. E3, Mamatha Ballal4, Sohan Bangera5, Mohandas Rao KG6 and Veena Nayak71Department of Pharmacology, Associate Professor, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India. 576104.
2Department of Pharmacology, Postgraduate, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India. 576104.
3Department of Pharmacology, Ph.D. Research Scholar, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India. 576104.
4Department of Microbiology, Professor, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India. 576104 .
5Department of Microbiology, Ph.D. Research Scholar, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India. 576104.
6Department of Anatomy, Professor and Head, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India. 576104.
7Department of Pharmacology, Associate Professor, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India. 576104.
Corresponding Author E-mail: abhijna.ballal@learner.manipal.edu
Abstract: The study was conducted to assess the effect of lignocaine and tramadol on healing of Staphylococcus aureus infected incision wound in rats. Incision wounds were created in three groups consisting of six rats in each group. The rats were infected with Staphylococcus aureus inoculum and the incision was closed. The rats were treated according to their group – Group I (Normal saline), Group II (2% Lignocaine) and Group III (5% Tramadol). All the drugs were applied topically, once a day for 10 days. The parameters measured to evaluate the wound healing was breaking strength, Colony Forming Units/ mL (CFU/ mL) of the tissue and histopathological examination. Data analysis was by one-way (Analysis of Variance) ANOVA followed by post hoc Tukey’s test. A significant difference (p<0.05 vs control) in the breaking strength and CFU/mL of tissue was observed between the control Group I and the test groups, Group II (2%lignocaine) and Group III (5% tramadol) when the drugs were used topically. Histopathological study did not show much difference in the morphology between the control and other groups, it revealed disorganised collagen fibres. This study suggests that lignocaine and tramadol do not impede healing of infected incision wound.
Keywords: Antibacterial; Breaking Strength; Colony Forming Units; Collagen; Incision; Staphylococcus Aureus Back to TOC