Effects of Carbon Sources on Cell Differentiation of Lactobacillus rhamnosus PN04 and Applications
Nguyen Tu Hoang Khue* and Doan Thi Thanh VinhSchool of Biotechnology, Hochiminh City International University, Hochiminh, Vietnam.
Abstract: Lactobacillus strainsare commonly used as probiotics. Formulation for the probiotics is essential. Thestudy has studied the effects of carbon sources including in glucose, fructose, maltose, lactose, saccharose at the different concentrations as 5, 10, 15, 20, 30, 40, 50 g/L on the cell differentiation of Lactobacillus rhamnosusPN04.As results, L. rhamnosusPN04formed differentiated cells those were called minicells with highly significantnumber as 7870minicells in one ml starting culture with the density of cells of 2.57×109 cellsin modified MRS broth with lactose 50 g/l.The minicells incubated with paclitaxel (10µg/mL) and cephalosporin (10µg/mL) in 10 hours were detectedon antimicrobial activities onStaphylococcusaureus ATCC 25923,Escherichia coli ATCC 9637, Salmonella typhiATCC 19430, Candidaalbicans ATCC 14053, Pseudomonasaeruginosa ATCC 27853.By scanning electron microscope, the minicell size was less than 400 nm. The study reported theL. rhamnosusPN04 differentiated cells produced in lactose containing MRS which will play a role in probiotic preparation and drug delivery.
Keywords: Lactobacillus rhamnosus; Cell differentiation; Carbon sources; Probiotic formulation; Drug package Back to TOC