The Potential of Basal Medium Astemporary Prolong Culture of Umbilical Cord Derived Mesenchymalstem Cells
Jeanne Adiwinata Pawitan1,2,3
, Tera Dria Kispa1, Isabella Kurnia Liem1,3,4, Fajar Mujadid1 and Novialdi Novialdi11Stem Cell Medical Technology Integrated Service Unit, Dr. Cipto Mangunkusumo General Hospital/Faculty of Medicine Universitas Indonesia, Jl. Diponegoro 71, Jakarta 10430, Indonesia.
2Department of Histology, Faculty of Medicine Universitas Indonesia, Jl. Salemba 6, Jakarta 10430, Indonesia.
3Stem Cell and Tissue Engineering Research Center, Indonesia Medical Education and Research Institute (IMERI), Faculty of Medicine Universitas Indonesia, Jl. salemba 6, Jakarta 10430, Indonesia.
4Department of Anatomy, Faculty of Medicine Universitas Indonesia, Jl. Salemba 6, Jakarta 10430, Indonesia.
Corresponding Author E-mail: jeanneadiwip@gmail.com
Abstract: To compare the viability and characteristics of umbilical cord mesenchymal stromal cells (UC-MSCs) when they were kept in culture in three types of αMEM after confluence for various time points. Viability and characteristic assessments were done after keeping UC-MSC confluent cultures for 0, 24, 48, 72, and 96hours in αMEM with glutamax (Himedia), and without glutamax (Himedia or Gibco).There were thirty cultures, which each ten of them were kept in the three types of basal media, and for each time point and type of basal medium, two flasks were harvested. All viability and characteristic assessments were done after harvest. Viability assessments were done in duplicate, while characteristics assessment by flowcytometry for CD73, CD90 and CD105 were done only once.Further, differences between the various time points in terms of viability and CD73, CD90 and CD105 percentage after various time points were compared and tested by appropriate statistical analysis. Viability was >70% upto 72 hours and 48 hours when the cells were kept in Himediaglutamax containing αMEM and αMEM without glutamax respectively. Flow cytometry showed that CD73, CD90 and CD negative percentage did not differ to initial percentage, but after 24 hours CD105 was slightly decreased to > 60% in Himediaglutamax containing αMEM, while in the two αMEM media without glutamaxthe percentages were below 40%. For our UC-MSC culture, glutamax containing αMEM is better compared to αMEM without glutamax as temporary storage solution, and storage should be restricted to 24 hours.
Keywords: Mesenchymal Stem Cell; Population Doubling Time; Storage Solution; Viability; Umbilical Cord Back to TOC