The Diagnostic Value of the P53 Tumor Marker as a Prognostic Factor in Patients with Squamous Cell Carcinoma of the Larynx
Saki Nader1*, Nikakhlagh Soheila1, Ranjbari Nastaran2, Shahrokhi Maryam31Associated of otolaryngology ,Hearing and Speech research Center, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran 2Assistant Prof. of Pathology, Cancer Research Center, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran 3Otolaryngologist, Cancer Research Center, Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran *Corresponding Author email : Saki-n@ajums.ac.ir
Abstract: The objective of this study was to determine expression of the P53 marker in cases of patients with squamous cell carcinoma of the larynx and also to study the possible relationship between the level of the marker and clinical prognostic and histopathologic factors of the disease. It was a prospective analytic epidemiologic study conducted on 42 patients diagnosed with squamous cell carcinoma of the larynx and subjected to laryngectomy. The paraffin blocks of the participants were examined and slides covered with anti-P53 antibodies were subjected to standard immunohistochemistry in order to find the antigen. To assess the level of the P53 marker, grades with scores less than 10% were considered to be negative. In addition, grades with scores between 10% and 60%, 61% and 79%, and 80% were also well-differentiated, moderately differentiated, and poorly differentiated, respectively. A total of 35 (83.3%) participants were P53-positive: 28.6% were supraglottic, 48.6% were glottic, and 22.8% were transglottic. In general, there was a significant difference between the level of the P53 marker and the extent and grade of tissue differentiation of the tumor, involvement of blood vessels, invasion of the peripheral nerves, invasion of extrinsic soft tissues of the larynx, distant metastasis. However, no significant difference was observed between the level of the P53 marker and the position of the tumor or involvement of lymph nodes. Surgical margins also showed no signs of positivity of the marker. The P53 marker is a valuable prognostic marker capable of revealing useful information on the clinical growth and position of carcinoma of the larynx.
Keywords: P53; Squamous Cell Carcinoma; Larynx; Immunohistochemistry Back to TOC