The Cytotoxic Effect of Methanolic Extract of Pyracantha coccinea M. Roemer Fruit on Hela cell line, Antioxidant Capacities and Total Phenol Contents of Methanolic and Aquatic Extract of this fruit
Leila Vahabi1, Ramesh Monajemi2, Seyed Ahmad Hosseini3*1Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. 2Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. 3Nutrition and Metabolic Diseases Research Center ,Ahvaz Jundishapur, University of Medical Sciences, Ahvaz, Iran *Corresponding Author: Email: seyedahmadhosseini@yahoo.com
Abstract: For years the plants as a natural resource to be used for the treatment of cancer. But if the development of anticancer drugs from natural sources such as plants needs to screening tests. This study aimed to assess the cytotoxic effect of Pyracantha coccinea fruit methanol extract on Hela cell line and evaluation of antioxidant properties and total phenolic content of methanol and aqueous extract of Pyracantha coccinea fruit was done. Hela cell culture medium RPMI-1640 containing 10% fetal bovine serum were cultured with 5% CO2. Fruit of Pyracantha coccinea, after being dried, solvent and aqueous was extracted with methanol by maceration method. Cytotoxic effect of the extract in different concentrations was evaluated by MTT assay at 72 hours. Pyracantha coccinea fruit was collected from Esfahan. Aqueous and methanol extracts preparation with Soaking method. Antioxidant activity was determined by scavenging of DPPH assay. Also this Pyracantha coccinea fruit extracts were analyzed for total phenolic contents with Folin-Ciocalteu reagent. Gallic acid was used as a standard compound and the total phenols were expressed as mg gallic acid /g dry extract equivalents. the cytotoxicity of the methanol extract at a concentration of 800 µg/ml was higher than other concentrations. The total phenolic content and antioxidant properties of the methanol extract was higher than Aqueous extract. The results indicate a weak cytotoxic effects of the methanol extract of Pyracantha coccinea fruit. Antioxidant properties of aqueous and methanol extracts of this plant compared to standard antioxidant BHT was very weak. Also, high levels of total phenolics content in the extracts were not observed. Such research can screening the effective and ineffective plant and extracts of different species plants and pave the way for the investigation of more than in the pharmacy.
Keywords: Fruit; Cytotoxic; Hela; Pyracantha Coccinea Back to TOC