Profiling of MicroRNA Expression within the Cells of Peripheral Blood Mononuclearafter an Infection with Serotype-2 of Dengue Virus: Preliminary Study

Sri Masyeni¹, Usman Hadi², K Kuntaman² and Yorapermata Dewi¹

¹Faculty of Medicine and Health Science, University of Warmadewa, JlTerompong 24, Denpasar-Bali,Indonesia.

²Faculty of Medicine, University of Airlangga, JlMayjen Prof. Dr. Moestopo 47, Pacar Kembang, Surabaya, Kota SBY, Jawa Timur, Indonesia.

Corresponding Author E-mail: masyeniputu@yahoo.com

Abstract: The role of microRiboNucleic Acids (miRNA), a small-non coding RNA has been associated with immune regulation in various viral infectionincluding dengue infection. The microRNA will bind a specific protein target in order to encourage an explosive expression of various cytokines, known as cytokines storm in Dengue infection.The objective of this study aimed to determine and evaluate themicroRNAs profile expression withinperipheral blood mononuclear cells having been infected with one of the dengue virus serotype.To obtained the PBMCs from a healthy donor, Ficoll density gradient centrifugation was used to isolate the PBMCs and then followed infecting it with a DENV-2 clinical isolate. Prior to PBMCs isolation, the virus has been propagated and having titration to get an optimal virus titer. We conducted the infection at the multiplication of infections 4 PFU/10⁶ cells.MiRCURYLNA^TMExiqon was utilized on purpose to extract the RNA. Quantitative Real-Time PCR was applied in order for the miRNAs relative expression to be measured. The preliminary result reveals that miR-150, miR-146a, hsa-let-7e expression were increased 1.74 folds, 2 folds, and 1.49 foldsrespectively at 12 hours post-infection on PBMCs upon DENV-2 infection.The expression of microRNAswas discovered to behigher inPBMCsat the time of infection withDENV-2.ThemiRNAs expression in the uninfected PMBCs was lower than that of the miRNA. This high expression of miRNAsin dengue infection may proceedto dengue infection pathogenesis.

Keywords: Dengue; Expression; Infection; MiRNA

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