Protective Effects of Curcumin in Formaldehyde Treated Lymphocytes and Cancer Cells and its Role in Superoxide Dismutase Activity
Malinee Pongsavee
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Rangsit Campus, Patumthani 12121, Thailand.
Corresponding Author E-mail: malineep@tu.ac.th
Abstract: Curcumin is a compound in Curcuma longa Linn. It has shown antioxidant and anti-inflammatory properties. Formaldehyde is the chemical compound with the formula H2CO. It is the toxic chemical substance and causes many types of cancer in human. The protective effects of curcumin in formaldehyde treated lymphocytes and cancer cells were investigated. The antimutagenic effect of curcumin was investigated in the formaldehyde treated lymphocytes. The antiproliferative effect of curcumin was investigated in cancer cells (MCF-7 cells, A549 cells and CRL-2724 cells). Role of curcumin in formaldehyde treated lymphocytes for SOD activity change was also studied. The formaldehyde concentrations of 0.036, 0.072, 0.15 mg/ml, 10 and 20 µM curcumin, Trypan blue exclusion assay, MTT assay, Giemsa staining, G-banding technique for chromosome investigation and SOD activity assay were used in this study. The results showed that curcumin has the protective effects of antimutagenicity and antiproliferation in formaldehyde treated lymphocytes and cancer cells. Curcumin effects SOD activity change in formaldehyde treated lymphocytes. Curcumin may apply in the therapy of diseases caused by the chemical substance toxicity.
Keywords: Curcumin; Cancer Cells; Formaldehyde Protective Effects; Lymphocytes; SOD Activity; Back to TOC