Taurine and Oyster Mushroom (Pleurotus Ostreatus) Prevents Oxidative Damage in Liver of Mice Induced by Paraquat
Bayu Putra Danan Jaya, Endang Linirin Widiastuti
, Endang Nurcahyani and SutyarsoDepartment of Biology, Faculty of Mathematic and Sciences, Universitas Lampung, Jalan Soemantri Brojonegoro No. 1, Gedung Meneng, Bandar Lampung, Lampung 35145, Indonesia.
Corresponding Author E-mail: elwidi@yahoo.com
Abstract: Paraquat is a toxic substance that can cause oxidative damage through increased ROS production. Oxidative damage can be prevented by supplementing with antioxidants, such as taurine and oyster mushrooms. This study aims to determine the ability of taurine and oyster mushrooms in preventing oxidative damage of the liver of mice induced by paraquat. This study uses a completely randomized design. A total of 30 DDY mice were divided into five treatment groups for 3 weeks, namely: 1) control, 2) the oyster mushroom (6.25% in feed and 2.5 g/L in drinking water), 3) paraquat (20 mg/kg, IP), 4) paraquat and taurine (15.6 g/kg) and 5) paraquat and oyster mushrooms. Parameters measured were MDA, glutathione, SOD enzyme levels and histopathological changes in liver. The results showed paraquat increases in liver MDA levels significantly but decreases in liver glutathione levels significantly compared to controls, while taurine and oyster mushrooms reduce the levels of MDA (p<0.05) and increase glutahion levels (p<0.05). Paraquat also increases the levels of SOD (p<0.05), while taurine and oyster mushroom are able to inhibit the increased levels of SOD although they do not show significant (p> 0.05). Paraquat induces liver histopathology change which is characterized by congestion, hydropic degeneration and cloudy swelling. In conclusion, paraquat causes oxidative damage to the liver, while taurine and oyster mushrooms can prevent the damage.
Keywords: Antioxidants; Oxidative Damage; Oyster Mushrooms; ROS and Taurine Back to TOC