Effect of Herbomineral Amino acid Formulation on Wistar Strain Diabetic Nephropathy Rats
Suresh Kumar Karri¹* and S. Srinivasan²¹Department of Pharmacology, Annamalai University, Chidambaram India.
²Department of Pharmaceutics, Krupanidhi College of Pharmacy, Bangalore - 560 035 India.
Abstract: The present study was aimed to investigate the single dose effects of a herbomineral amino acid extract (HMAA) of Spinacia oleracea on alloxan induced diabetic nephropathy rats. In the present study, the animals were divided into 6 groups Group 1 – Normal saline as vehicle –control, Group 2 – Untreated diabetic rats (Alloxan induced), Group 3 – Animals treated with Metformin (0.5 mg/kg) – standard drug, Group 4 – Animals treated with Extract alone, Group 5 – Animals treated with Herbal + Mineral, Group 6 – Animals treated with Herbal + Mineral + Amino Acid. Based on the oxidative stress hypothesis of alloxan action, the role of herbal extract on free radicals in the pathology of diabetes mellitus has been studied in diabetic nephropathy rats. The present study demonstrated that herbal extract in the presence of an aminoacid (L-Arginine, 0.1 mM) and mineral (Zn+, in the form of Zinc Sulphate, 0.5 mM) could be a potent antioxidant and further, the data suggested that the mechanism underlying such protection is mediated via restoration of diabetic nephropathy leading to normalization of the diabetic rats.
Keywords: Spinacia oleracea; HMAA; anti-oxidant; diabetic nephropathy Back to TOC