Determination of Microbial Load in Reference to Stability Study of Churnas (powders)

Niranjan Sonkar¹, Alka Agrawal², Gopal Nath³ and C. B. Jha¹

¹Department of Rasa Shastra, Institute of Medical Sciences, Banaras Hindu University, Varanasi - 221 005 India. ²Department of Medicinal Chemistry, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi - 221 005 India. ³Department of Microbiology, Faculty of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi - 221 005 India.

Abstract:

The aim of broth and agar dilution methods is to determine the lowest concentration of the assayed antimicrobial agent (minimal inhibitory concentration, MIC) that, under defined test conditions, inhibits the visible growth of the bacterium being investigated. MIC values are used to determine susceptibilities of bacteria to drugs and also to evaluate the activity of new antimicrobial agents¹. For the present study the trial drugs taken i.e. Haritaki Churna, Vibhitaki Churna, Amalaki Churna and Triphala Churna retain their potency i.e. no deterioration is observed in terms of physical, chemical and microbiological parameters after one month, two months, four months and six months duration in the Stability chambers - Temperature 450C ± 2 & Relative Humidity 75% ± 5 and in ordinary conditions of temperature and humidity. This shelf life period may be applicable to other Churnas having similar method of preparation and constituents having similar range of phyto-chemicals, carbohydrates, cellulose etc.

Keywords:

Hc. (Haritaki churna); Vc. (Vibhitaki churna); Ac. (Amalaki Churna); Tc. (Triphala churna)

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