Screening of B-Galactosidase Producing Fungi from Marine Samples
Pavani Anumukonda* and Prabhakar Tadimalla
1Pharmaceutical Biotechnology Division, Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam - 530 003 India.
Abstract:
Fungi are used in many industrial processes, such as the production of enzymes, vitamins, polysaccharides, polyhydric alcohols, pigments, lipids, and glycol-lipids. Some of these products are produced commercially while others are potentially valuable in biotechnology. Fungal secondary metabolites are extremely important to our health and nutrition and have tremendous economic impact. The screening and isolation procedures resulted, five potentially interesting fungal isolates (MF S2-02, MF S3-09, MF S4-11, MF S5-08, and MF S6-09). All were able to produce more then 50 U/gm DCW of intracellular activity and 10 U /ml extracellular activity. The results indicate that the isolate MF S4-11 is promising strain with the yield of 117 U/gm DCW. A good number of media that were reported in the literature were screened to select optimal production media and medium number ‘IV’ was found to be the best for the production of â-galactosidase (128 U/ gm DCW).
Keywords:b-galactosidase; lactose hydrolysis; transgalactosylation; marine samples
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