Ethanol Extract of Annona Muricata. L Induces Liver Cancer Cell Apoptosis Through ROS Pathway
Huali Yang, Na Liu and Shaochin LeeSchool of Life Sciences, Shanxi University, Taiyuan, Shanxi, PR China.
Corresponding Author E-mail: lee_shao@hotmail.com
Abstract: The study investigated the underlying molecular mechanisms of ethanol extract of Annona muricata. L induced liver cancer cell apoptosis. The anti-proliferative effect of ethanol extract of Annona muricata. L was evaluated by MTT assay. Flow cytometry was used to detect the cell cycle and apoptosis. TUNEL assay was used to confirm cell apoptosis. DCFH-DA fluorescent probe can detect the level of intracellular reactive oxygen species (ROS). Different concentrations of ethanol extract of Annona muricata. L leaves (0, 30, 60, 120, 240 µg/ml) were used to treat liver cancer HepG2 cells for 24h or 48h, the cell viability was inhibited in time- and dose-dependent manners. Ethanol extract of Annona muricata. L leaves increased the number of cells in the sub-G1 fraction in a dose-dependent manner. TUNEL assay confirmed that the extract caused apoptosis of the cancer cells. Further analysis revealed that the extract produced reactive oxygen species (ROS). Antioxidant N-acetylcysteine was able to inhibit the extract-triggered ROS production and apoptosis. In conclusion, our results suggest that the ethanol extract of Annona muricata. L leaves induces HepG2 cell apoptosis through ROS pathway.
Keywords: Annona muricata. L; liver cancer cell; apoptosis; ROS Back to TOC