Investigating the inhibitory effects of hydro alcoholic extracts of Aloe Vera, Anethum graveolens and Kelussia Odoratissma on acid phosphatase derived from rat liver.
Mohammad Aberomand1, Mohammad Ebrahim Azemi2* and Sima Rashidi31Department of Biochemistry, School of Mendicant, Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences. Ahvaz, Iran. 2Department of Pharmacognosy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. 3Department of Medicinal Chemistry, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences. Ahvaz, Iran.
Abstract: Acid phosphatase called orthophosphoric-monoester phosphohydrolase (Ec: 3.1.3.2) is produced by plants and microbes and is a group of enzymes which catalyze hydrolysis of a variety of orthophosphate esters reactions in acid medium. This study aims to comparatively investigate the inhibiting effect of the three extracts Aloe Vera, Anethum graveolens, and Kelussia Odoratissma on the activity of acid phosphatase. This was an experimental in vitro study. Acid phosphatase extracted from rat liver was partially purified by successive chromatography based separating technique on the sephadex G50. This enzyme was purified and its final specific activity was 479.35 U/mg. The inhibiting effect of the three extracts on the activity of acid phosphatase was determined. All three tested plant extracts inhibited the activity of the enzyme acid phosphatase. Aloe Vera showed the most inhibiting value (82.57%), follwed by Kelussia Odoratissma (5.6%) and Anethum graveolens (4.07%). The results of this study show that all Aloe Vera can extract the acid phosphatase enzyme activity, and reduce plant extracts.
Keywords: Enzyme Acid phosphatase; Aloe Vera; Anethum graveolens; Kelussia Odoratissma; Inhibiting Back to TOC