Anti-Inflammatory and Antioxidant Properties of the Mucuna sanjappae Seeds in the Rat Model and In Vitro Assays
Ravishankar Patil1,2,4,5, Chetan Aware1, Kavita Shinde3,6, Ruchika Kaul-Ghanekar3,6,7, Govind vyavahare1, Vishwas Bapat1 and Jyoti Jadhav1,2*1Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, Maharashtra, India.
2Department of Biochemistry, Shivaji University, Vidyanagar, Kolhapur, Maharashtra, India.
3Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth University, Pune, Maharashtra, India.
4Amity Institute of Biotechnology, Amity University, Mumbai, Maharashtra, India.
5Amity Centre for Nuclear Biotechnology, Amity University, Mumbai, Maharashtra, India
6Cancer Research Lab, Symbiosis School of Biological Sciences (SSBS), Symbiosis International (Deemed University), Pune Maharashtra, India
7Symbiosis Centre for Research and Innovation (SCRI), Symbiosis International (Deemed University), Pune, Maharashtra, India
Corresponding Author E-mail:profjpjadhav@gmail.com
Abstract: The Fabaceae (Leguminosae) plant family contains several species of the Mucuna Adans. genus possessing therapeutic potential and growing widely in tropical and sub-tropical regions. In this research, we investigated the anti-inflammatory and antioxidant properties of the extract from the Mucuna sanjappae Aitawade & S.R.Yadav seeds. Initially, we conducted an in vitro anti-inflammatory activity test using the bovine serum albumin anti-denaturation assay and found promising dose-dependent activity. Subsequently, we performed an in vivo anti-inflammatory and antioxidant study on a rat paw edema model induced by carrageenan. Three different doses of M. sanjappae seed water extract (50, 100 and 200mg/kg B/W) were used for the study (Oral administration). Edema measurement was carried out at 0, 2, 4 and 6 hr intervals. Dose dependent inhibition in edema in the M. sanjappae seed extract treatment group was observed with maximum activity for 200mg/kg B/W dose at 4 hr (53.49%). Standard drug showed maximum edema inhibition (54.94%) at 6hr. Our results also showed that, M. sanjappae seed extract inhibited pro-inflammatory cytokine TNF-α and increases anti-inflammatory cytokine IL-10 with increased level of blood serum antioxidants. Phytochemical analysis for secondary metabolites including polyphenol, flavonoids, phytic acid, proanthocyanidin, tannin and saponin was also quantified which might be the responsible component for biological activities under study.
Keywords: Antioxidant; Anti-inflammatory drug; Carrageenan; Cytokine; Inflammation; Mucuna Back to TOC